The Fluoro H202 detection kit utilizes a non-fluorescent detection reagent, to detect H202. H202 oxidizes the detection reagent in a 1:1 stoichiometry to produce a fluorescent product resorufin. This oxidation is catalyzed by Peroxidase in a homogeneous no wash assay system.
The detection reagent can be utilized to detect H202 release from cells or enzyme coupled reactions (1-7)
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