• 产品编号:FLOH 100-3
    • 产品名称:Fluorescent Hydrogen peroxide/Peroxidase Detection Kit/荧光过氧化氢/过氧化物酶检测试剂盒
    • 产品规格:500 Tests
    • 价  格:3375
    • 供 应 商:Cell Tech
    • 库  存:
  • Fluoro H202
    Fluorescent Hydrogen peroxide/Peroxidase Detection Kit
     

    Key Benefits:
    • Quick 10 minute assay
    • Can monitor multiple time points to follow kinetics.
    • Dual mode, can detect H202 or peroxidase activity.
    • One-step, no wash assay.
    • Adaptable for High Throughput format
    • Non-destructive cell based assay allows monitoring of additional parameter
    • Applications - Fluorescent Plate Reader
    Assay Principle

    The Fluoro H202 detection kit utilizes a non-fluorescent detection reagent, to detect H202. H202 oxidizes the detection reagent in a 1:1 stoichiometry to produce a fluorescent product resorufin. This oxidation is catalyzed by Peroxidase in a homogeneous no wash assay system.

    The detection reagent can be utilized to detect H202 release from cells or enzyme coupled reactions (1-7)

    Reaction:
                                                                peroxidase
    H202 + detection reagent (non-fluorescent) --------->   Resorufin
                                                                                (fluorescent)
     
    Citations
    Deficiency of the Bax gene attenuates denervation-induced apoptosis - P. M. Siu and S. E. Alway - Apoptosis Journal Vol 11/No 6 June 2006
    References
    1. Anal Biochem 253, 162 (1997);
    2. J. Immunol Methods 202, 133 (1997);
    3. J Neurochem 79, 266 (2001);
    4. Am J Physiol Lung Cell Mol Physiol 281, L993 (2001);
    5. Mol Hum Reprod 7, 237 (2001);
    6. Anal Biochem 287, 196 (2000);
    7. J Invest Dermatol 112, 751 (1999).
    Kit contents (for 500 assays)
    1. 5X Reaction Buffer: 20 ml buffer, pH 7.4
    2. Detection reagent: One vial for 500 assays.
    3. Hydrogen Peroxide: 200µL of a stabilized 3% solution.
    4. Horseradish Peroxidase: 18.9 Units of enzyme
       
    The following kits are available:
    Catalog #
    Size
    Price 
    FLOH 100-3
     
    500 Tests 3375.00

    荧光过氧化氢/过氧化物酶检测试剂盒

    主要优点:

    10分钟快速检测。

    遵循动力学,检测多个时间点。

    双模式,可以同时检测过氧化氢或过氧化物酶的活性。

    一步到位,免洗。

    适应高通量检测模式。

    不损伤细胞,可以检测更多的参数。

    应用 - 荧光酶标仪

     

    检测原理:

    荧光过氧化氢检测试剂盒采用了非荧光检测试剂,用来检测过氧化氢。过氧化氢氧化的检测试剂以1:1的比例混合,生产荧光产物9-羟基-3-异吩-唑酮。这种氧化反应是在过氧化物酶催化下的均质免洗检测系统中进行的。

    该检测试剂可以用来检测细胞或酶偶联反应(1-7)中过氧化氢的释放。

    Reaction:

                                                过氧化物酶

    H202 +检测试剂 (非荧光)  --------->   9-羟基-3-异吩-唑酮(荧光)

    激发波长:530-571nm

    发射波长: 590-600nm