• 产品编号:AChE 100
    • 产品名称:Fluorescent Acteylcholinesterase (AChE) Detection Kit - Detection of AChE in RBC, Saliva, and Tissue Lysates/荧光乙酰胆碱酯酶检测试剂盒-可检测红细胞、唾液和组织裂解液
    • 产品规格:100Test/500Test
    • 价  格:5925/23925
    • 供 应 商:Cell Tech
    • 库  存:
  • Fluoro AChE™
    Fluorescent Acteylcholinesterase (AChE) Detection Kit - Detection of AChE in RBC, Saliva, and Tissue Lysates
     
    Key Benefits:
    • Non Radioactive assay can monitor multiple time points to follow kinetics.
    • One-step, no wash assay
    • Versatile: Can Detect AChE activity in RBCs, Saliva and Tissue Lysates
    • Readout - 96 well Fluorescence Plate Reader
    Introduction
    Exposure to chemical nerve agents, pesticides and certain drugs (anesthetics, cocaine and therapeutical drugs) reduces the activity of red blood cell (RBC) acetylcholinesterase (AChE). The RBC-AChE can be used as a biomarker to monitor suppressed and or increased AChE function in the peripheral and central nervous system (9).
    Acetylcholinesterase (AChE) is one of the most important enzymes involved in nerve transmission. The enzyme is bound to cellular membranes of excitable tissue (synaptic junction, endoplasmic reticulum, etc) 1-3. Acute toxicity to humans and animals through inhibition of AChE by both nerve gases and an important class of pesticides has long been a field of intensive scientific investigation 4,5. AChE inhibitors have also been used clinically as Alzheimer’s treatments (e.g., tacrine (tetrahydroaminoacridine)) 6 and are the subject of increasing interest in various disease processes and treatment strategies 7,8. However, both environmental detection of AChE inhibitors and development of modulators of AChE enzymatic activity as drugs have been hampered by the difficulty and complexity of the current assay methods.
     
    Assay Principle
    We have developed a highly sensitive, very rapid, extremely simple assay to determine acetylcholinesterase activity in RBC’s, using the natural substrate, acetylcholine. Additionally, by using specific inhibitors, the kit can be used to detect AChE activity in a variety of samples. A series of coupled enzyme reactions quickly translates the presence of active AChE into a change in the fluorescence of a quenched detection reagent.
    AChE + ATP + H20 + coupled enzyme reaction + quenched dye ----> Fluorescent  
                                                                                                            Dye (Ex:530-570nm 
                                                                                                                   Em:590-600nm 
    References
    1. Politoff, A., Blitz, A., and Rose, S.: Incorporation of Acetylcholinesterase Into Synaptic Vesicles is Associated with Blockade of Synaptic Transmission, Nature 256, 324, 1975
    2. Friedenberg, R., and Seligman, A.: Acetylcholinesterase at the Myoneural Junction: Cytochemical Ultrastructure and Some Biochemical Considerations, J Histochem Cytochem 20, 771, 1972
    3. Nachmansohn, D.: Proteins in Excitable Membranes, Science 168, 1059, 1970.
    4. HA Berman and MM Decker. Kinetic, equilibrium, and spectroscopic studies on dealkylation ("aging") of alkyl organophosphonyl acetylcholinesterase. Electrostatic control of enzyme topography. J. Biol. Chem., Aug 1986; 261: 10646-10652 .
    5. Arie Ordentlich et al. The Architecture of Human Acetylcholinesterase Active Center Probed by Interactions with Selected Organophosphate Inhibitors. J. Biol. Chem., May 1996; 271: 11953-11962.
    6. Levy R. Tetrahydroaminoacridine and Alzheimers disease. Lancet, 1987 Feb 7;1(8528):322.
    7. Bolognesi ML et al. Propidium-based polyamine ligands as potent inhibitors of acetylcholinesterase and acetylcholinesterase-induced amyloid-beta aggregation. J Med Chem. 2005 Jan 13;48(1):24-7.
    8. Schallreuter KU et al. Activation/deactivation of acetylcholinesterase by H2O2: more evidence for oxidative stress in vitiligo. Biochem Biophys Res Commun. 2004 Mar 5;315(2):502-8.
    9. Nigg HN, Knaak JB. Blood cholinesterases as human biomarkers of organophosphorus pesticide exposure. Rev. Environ. Contam. Toxicol, 2000:163: p29-111.
    Kit contents (for 100 assays)
    1. Component A: Detection Reagent Diluent, 5.5mL.........Part# 3023
    2. Component B: Coupled enzyme reagent, 5.5mL..........…Part# 3024
    3. ComponentC: 1vial detection reagent………………………………Part# 4016
    4. Component D: 5X Reaction Buffer………………………………………Part# 3011
    5. Component E: Acetylcholine…………………………………………………Part# 7012
    6. Component E: Red Blood Cell Acetylcholinesterase………Part# 6020
    The following kits are available:
    Catalog #
    Size
    Price (RMB)
    AChE 100-2 100 Tests 5925.00
    AChE 100-3 500 Tests 23925.00

    Fluorescent Acteylcholinesterase (AChE) Detection Kit - Detection of AChE in RBC, Saliva, and Tissue Lysates/荧光乙酰胆碱酯酶(红细胞,唾液,组织裂解液)检测

    主要优点:

    非放射性检测,遵循动力学,可以检测多个时间点。

    一步法,免洗。

    多功能:可以检测红细胞,唾液,组织裂解液中的乙酰胆碱酯酶。

    应用:96孔荧光酶标仪。

     

    介绍:

    暴露在化学神经毒剂,杀虫剂和某些药物(麻醉剂,可卡因和治疗药物)能够降低红细胞(RBC)中乙酰胆碱酯酶(AChE)的活性。红细胞-乙酰胆碱酯酶可作为一种生物标志物来监测周边和中枢神经系统的被抑制的或增加的乙酰胆碱酯酶的功能(9)。

     

    乙酰胆碱酯酶(AChE)是神经传递所涉及的最重要的酶之一。这种酶结合到兴奋组织(突触交界处,内质网等)的细胞膜上1-3。抑制乙酰胆碱酯酶对人类和动物的急性毒性,一直在进行深入的科学考察4,5。乙酰胆碱酯酶抑制剂也被用于阿尔茨海默病的临床治疗6,并且是增加的各种疾病和治疗策略所关注的课题7,8。然而,无论乙酰胆碱酯酶抑制剂,用于环境检测,还是乙酰胆碱酯酶活性调节剂作为药物的研发,都受到当前检测方法的复杂性的阻碍。

     

    检测原理:

    我们研发了一种高敏感,快速,非常简单的方法检测红细胞中乙酰胆碱酯酶的活性,通过使用天然底物,乙酰胆碱来检测。而且,使用特异性抑制剂,可以用试剂盒检测各种样品中的乙酰胆碱酯酶。一系列的配对酶反应可以快速的将活性乙酰胆碱转变成荧光检测试剂。

    AChE + ATP + H20 + 配对酶反应 + 染料 ----> 荧光产物

                                                                                                           

    激发波长:530-570nm   发射波长:590-600nm