Product code  NRaS

Physical form  Delipidated, lyophilized, stable reference serum of several rat
strains

Preservative  No preservative added

Intended use  As  a  qualitative  serum  with  an  optimized  protein  content  to
study  the normal protein  composition of  rat  serum. To  identify or quantitate a normal serum protein component using a variety of immunodiffusion techniques including immunoelectrophoresis, single  and  double  radial  immunodiffusion  and  electroimmuno-diffusion.  As  a  reference  serum  in  nephelometry  and  other
automated precipitation techniques. As a blocking agent or as a negative  control  in  non-precipitating  antibody-binding  assays e.g.  in  serodiagnostic  immunofluorescence  and  immunoenzyme tests.
This  normal  reference  serum  does  not  contain  assigned  values for  individual  proteins  but  can  be  used  as  an  internal  relative standard  for  quantitative protein  assays  such  as  double  radial immunodiffusion  (Mancini,  Fahey),  ELISA,  Western  immuno-blotting  and  electroimmunodiffusion  (Laurell),  expressing  the results obtained with the serum sample under investigation as a
percentage of the protein concentration in the normal reference serum. For a  standard  rat  serum with assigned values  for  IgA, IgM and the subclasses of IgG see NOR-03.

Directions for use  Normal serum protein  reference  sera are shipped  lyophilized at ambient  temperature.  After  arrival  store  at  +4°C.  Prolonged storage may be at  -20°C. After opening the vial, the lyophilized content is reconstituted by adding 1 ml of sterile distilled water, mixed gently by inversion until complete dissolution is obtained. Allow  to  stand  at  ambient  temperature  for  5-10  minutes  to reach  equilibrium.  Reconstituted  serum may  be  stored  frozen.
Working  dilutions  may  be  prepared  by  adding  the  required amount  of  phosphate  buffered  saline  (PBS),  should  not  be refrozen and preferably used within 24 hours. Repeated thawing and  freezing  should be  avoided.  If  a  slight precipitation  occurs upon storage, this should be removed by centrifugation and will not affect the performance of the reference serum.

Packing  Vial with 1 ml lyophilized normal protein reference serum.

Storage / shelf life