• 产品编号:EL-DF2
    • 产品名称:Der f 2 ELISA kit (EL-DF2)
    • 产品规格:/
    • 价  格:12720.00
    • 供 应 商:Indoor
    • 库  存:
  • Description: ELISA kit for measuring D. farinae allergen, Der f 2.
    Antibodies:
    1D8/7A1
    Sensitivity:
    0.5ng/ml
    Limit of Detection: 2ng/ml
    Contents:
    Monoclonal antibody 1D8, Der f 2 Standard, Biotinylated antibody 7A1; sufficient reagents for 20 plates.
    Storage: Store at 4°C
    Not Provided:
    50mM Carbonate/Bicarbonate buffer, pH 9.6
    Phosphate Buffered Saline, pH 7.4, containing 0.05% Tween 20 (PBS-T)
    Bovine Serum Albumin
    Streptavidin-Peroxidase
    1mM ABTS in 70mM citrate-phosphate buffer, pH4.2
    Notes:
    Product Resources: EL-DF2 Certificate of Analysis
    Related Products:
    For Research Use Only: Not for Diagnostic or Therapeutic Use
    Certificate of Analysis
    Monoclonal Antibody:
    1D8 (clone 1D8 C4 D8)
     
    Vial: Red Cap; 100μl
    Immunogen: Der p 2
    Isotype: Mouse IgG1
    Specificity:
     
    Binds to a common epitope on mite Dermatophagoides spp. Group 2 allergens, Der p 2 and Der f 2. 
     
     
    Purification:
    From ascites by ammonium sulphate precipitation and by HPLC using recombinant protein G. Single heavy and light chain bands on SDS-PAGE
    Concentration:
    1.0 mg/ml in phosphate buffered saline, pH 7.4 Based on A280 for IgG (1.42 = 1mg/ml). 0.22μm filtered, preservative free.

    Certificate of Analysis
    Monoclonal Antibody:
    Biotin 7A1 (clone 7A1 H11 G3)
    Vial: Brown Cap; 100μl
    Immunogen: Der f 2
    Isotype: Mouse IgG1
    Specificity:
    Binds to a common epitope on mite Dermatophagoides spp Group 2 allergens (Der f 2 and Der p 2). 
    Purification:
    From ascites by ammonium sulphate precipitation and by HPLC using recombinant protein G. Single heavy and light chain bands on SDS-PAGE.  
    Biotinylation:
    Biotinylated using EZ-Link Sulfo-NHS-LC Biotinylating Agent and titrated for use in ELISA at 1/1000 dilution.Prepared in 1% BSA/50% glycerol/PBS, pH 7.4, 0.22μm filtered, preservative free. 

    Certificate of Analysis
    Allergen Standard:
    Natural Der f 2
    Vial: White Cap; 400μl/vial
    Composition:
    Prepared in 1% BSA/50% glycerol/PBS, 0.22μm filtered, preservative free, pH 7.4.
    Concentration:
    2500 ng/ml Der f 2
    Calibration:
    Natural Der f 2 >95% pure by SDS-PAGE analysis. Protein concentration determined by amino acid analysis.

     

     

     

     
    References:
    (For complete reference list see EL-DF2 Certificate of Analysis)