• 产品编号:CYT-320
    • 产品名称:重组小鼠可溶性RANK配体/sRANKL Mouse
    • 产品规格:2μg/10μg/1mg
    • 价  格:700.00/1800.00/
    • 供 应 商:ProSpecbio
    • 库  存:
  • Catalogue Number CYT-320
    Synonyms Soluble Receptor Activator of NFkB Ligand, TNFSF11, TRANCE, TNF-related activation-induced cytokine, OPGL, ODF, Osteoclast differentiation factor, Tumor necrosis factor ligand superfamily member 11, Receptor activator of nuclear factor kappa B ligand, RANKL, Osteoprotegerin ligand, CD254 antigen, sRANKL, sOdf.
    Introduction RANKL binds to tnfrsf11b/opg and to tnfrsf11a/rank. Osteoclast differentiation and activation factor. augments the ability of dendritic cells to stimulate naive t-cell proliferation. May be an important regulator of interactions between t-cells and dendritic cells and may play a role in the regulation of the t-cell-dependent immune response. sRANKL may also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy.
    Description sRANKL Mouse Recombinant produced in E.coli is single, non-glycosylated, polypeptide chain containing 174 amino acids and having a total molecular mass of 19.9kDa.
    CD254 is purified by proprietary chromatographic techniques.
    Source Escherichia Coli.
    Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.
    Formulation The protein (1mg/ml) was lyophilized with 10mM NaP, pH 7.5+ 50mM NaCl.
    Solubility It is recommended to reconstitute the lyophilized sRANKL in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
    Stability Lyophilized TNFSF11 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution sRANKL should be stored at 4°C between 2-7 days and for future use below -18°C.
    For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
    Please prevent freeze-thaw cycles.
    Amino acid sequence PAMMEGSWLD VAQRGKPEAQ PFAHLTINAA SIPSGSHKVT LSSWYHDRGW AKISNMTLSN GKLRVNQDGF YYLYANICFR HHETSGSVPT DYLQLMVYVV KTSIKIPSSH NLMKGGSTKN WSGNSEFHFY SINVGGFFKL RAGEEISIQV SNPSLLDPDQ DATYFGAFKV QDID.
    Purity Greater than 95.0% as determined by(a) Analysis by RP-HPLC.
    (b) Analysis by SDS-PAGE.
    Biological Activity Measured by its ability to induce osteoclast formation on murine RAW264.7 cells using a concentration of 5.0-10.0 ng/ml, corresponding to a specific activity of 100,000-200,000Units/mg.
    Usage ProSpecs products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.