• 产品编号:FAB200
    • 产品名称:Antibody Specific Active Caspase 3 检测试剂盒
    • 产品规格:25Test/100Test
    • 价  格:2325/6675
    • 供 应 商:Cell Tech
    • 库  存:
  • APO ACTIVE 3 ™
    Antibody Specific Active Caspase 3 Detection Kit

    Key Benefits:
    • Highly specific for active human and murine caspase 3. Other assays require the utilization of peptide based (DEVD) reagents that tend to cross-react with caspase 7 and other caspases.
    • Applications  - Works with a fluoresence microscope, 96 well plate reader, or flow cytometer.
    • Yields both quantitative and qualitative results. Gives a strong positive signal.
    • Can be used in conjunction with other antibodies or stains.
    • No need to make cell lysates or run western blots. Cells can be fixed and analyzed later.
    Assay Principle

    Cell Technology ’s APO-ACTIVE CASPASE 3 Kit utilizes a rabbit affinity purified polyclonal antibody raised against amino acid 163-175 of murine caspase 3 (9). This neo epitope is present on the p18 subunit of cleaved caspase 3 (9). Cells undergoing apoptosis are fixed and permeablized prior to the addition of the antibody. A secondary FITC labeled goat anti rabbit antibody is used to visualize the bound rabbit anti caspase 3 polyclonal.

    Citations

    The endoplasmic reticulum is the site of cholestrol-induced cytotoxicity in macrophages - Feng, Yao, Li, Devlin et al - Nature Cell Biology Vol 5, No 9, September 2003, pp 781-792

    Deficiency of the Cyclin Kinase inhibitor p21(WAF-1/CIP-1) Promotes Apoptosis of Activated/Memory T Cells, and inhibits spontaneous systemic Autoimmunity - J. Exp Medicine; Vol 199, Number 4, Feb 16 2004; 547-557

    Melanin acts as a potent UVB photosensitizer to cause an atypical mode of cell death in murine skin - Seiji Takeuchi, Wengeng Zhang, Kazumasa Wakamatsu, Shosuke Ito,Vincent J. Hearing, Kenneth H. Kraemer and Douglas E. Brash - PNAS U S A. 2004 October 19; 101(42): 15076–15081

    Enhanced growth inhibition of squamous cell carcinoma of the head and neck by combination therapy of paclitaxel and opioid growth factor - Patricia McLaughlin, Jeffrey Jaglowski, et al - International Journal of Oncology 26: 809-816, 2005

    Maternal Antibody Responses to the 52-kd SSA/Ro p200 Peptide and the Development of Fetal Conduction defects - Clancy, Buyon, Ikeda, et.al - Arthritis and Rheumatism Vol 52, No 10, October 2005, pp 3079-3086

    Combination chemotherapy with gemcitabine and biotherapy with opioid growth factor (OGF) enhances the growth inhibition of pancreatic adenocarcinoma - Ian S. Zagon, Jeffrey R. Jaglowski, et.al. -  Cancer Chemotherapy and Pharmacology – Vol 56/ No 5, Nov 2005

    References
    1. Slee, E. A., C. Adrain, and S. J. Martin. 1999. Serial Killers: ordering caspase activation events in apoptosis. Cell Death and Differ. 6:1067-1074.
    2. Walker, N. P., R. V. Talanian, K. D. Brady, L. C. Dang, N. J. Bump, C. R. Ferenz, S. Franklin, T. Ghayur, M. C. Hackett and L. D. Hammill. 1994. Crystal Structure of the Cysteine Protease Interleukin-1ß-Converting Enzyme: A (p20/p10)2 Homodimer. Cell 78:343-352.
    3. Wilson, K. P., J. F. Black, J. A. Thomson, E. E. Kim, J. P. Griffith, M. A. Navia, M. A. Murcko, S. P. Chambers, R. A. Aldape, S. A. Raybuck, and D. J. Livingston. 1994. Structure and mechanism of interleukin-1 beta converting enzyme. Nature 370: 270-275.
    4. Rotonda, J., D. W. Nicholson, K. M. Fazil, M. Gallant, Y. Gareau, M. Labelle, E. P. Peterson, D. M. Rasper, R. Ruel, J. P. Vaillancourt, N. A. Thornberry and J. W. Becker. 1996. The three-dimensional structure of apopain/CPP32, a key mediator of apoptosis. Nature Struct. Biol. 3(7): 619-625.
    5. Kumar, S. 1999. Mechanisms mediating caspase activation in cell death. Cell Death and Differ. 6: 1060-1066.
    6. Alnemri, E.S. et al (1996) Cell 87:171
    7. Trends Biochem Sci 22,388 (1997)
    8. Nature 376, 37 (1995).
    9. Srinivasan A, et al Cell Death and Differentiation (1998) 5: 1004-1016
    Kit contents
    • 1 vial of Rabbit anti cleaved caspase 3 affinity purified polyclonal antibody
    • 1 vial of FITC labeled Goat anti Rabbit affinity purified polyclonal antibody
    • 1 vial of 10X fixative.
    The following kits are available:
    Product
    Catalog No.
    Size
    (No. of Tests)
    Price (US$)
    APO ACTIVE 3 Antibody Specific Active Caspase 3 Detection Kit FAB200-1 25 2325.00
    FAB200-2
     
    100 6675.00

    APO ACTIVE 3 Antibody Specific Active Caspase 3 Detection Kit

    抗体特异性活性半胱天冬酶3检测试剂盒

    主要优点:

    高特异性的活性人和鼠的半胱天冬酶3。其他实验需要利用DEVD试剂,并且与半胱天冬酶7和其他半胱天冬酶进行交叉反应。

    应用 - 适用于流式细胞仪,荧光显微镜,或96孔板酶标仪。

    定量和定性结果。有强烈的阳性信号。

    可用于与其他抗体或染料结合。

    不需要裂解细胞液或进行免疫印迹分析。细胞可以被固定,然后进行分析。

    应用于人类,小鼠和大鼠细胞系。

     

    检测原理:

    Cell Technology APO-ACTIVE 3 PE试剂盒应用了抗鼠半胱天冬酶3163-175氨基酸肽段的亲和纯化兔多克隆抗体。新的抗原表位在裂解的半胱天冬酶39P18亚基上。发生凋亡的细胞在抗体增加之前是被固定的,并且是可细胞渗透的。PE标记的羊抗兔第二抗体可结合到兔抗caspase 3多克隆抗体上。PE标记可用于各种实验中。