• 产品编号:具体见表格
    • 产品名称:IMAC Phosphate & Elution Buffer Tablets
    • 产品规格:具体见表格
    • 价  格:具体见表格
    • 供 应 商:Medicago
    • 库  存:
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    Features 
    • Dissolve and go
    • Exactly pre-weighed tablets in bottles
    • Mix elution buffer with phosphate buffer to achieve desired imidazole concentration
    • Ultrapure, low UV280 absorbance imidazole
    • Formulated from analytical grade chemicals

     
    Product description
    Immobilized metal ion affinity chromatography (IMAC) is a widely used separation method for purifying proteins and peptides that have an affinity for metal ions, such as histidine-tagged proteins but also some untagged recombinant or native proteins. Medicago’s IMAC phosphate and elution buffer tablets have been developed exclusively for use in this method. 
    IMAC buffer tablets are supplied in bottles and in blister packs. One tablet of phosphate buffer dissolved in deionized water yields a 300 mM sodium chloride, 50 mM sodium phosphate buffer with pH 8.0 at 22°C. One tablet of elution buffer yields a 300 mM sodium chloride, 300 mM imidazole, 50 mM sodium phosphate buffer with pH 8.0 at 22°C.
     
    Applications
    • Purification of histidine-tagged and non-histidine-tagged recombinant and native proteins using IMAC chromatography
    • The phosphate buffer may be used for other types of affinity chromatography methods
     
    Directions for use
    Prepare the phosphate and the elution buffers separately. Deposit one tablet in a laboratory flask or beaker placed on a magnetic stirrer. Add deionized water up to the given volume for each tablet and stir until full dissolution. Mix the phosphate buffer and elution buffer to achieve desired imidazole concentration, follow the dilution series included in the product sheet.
     
    Shipping and storage
    IMAC phosphate and elution buffer tablets are shipped at room temperature. Store the tablets in a dry place at room temperature. Shelf life for IMAC phosphate buffer tablets is five years and shelf life for IMAC elution buffer tablets is three years.
     
    Specifications
    Chemicals: Analytical grade
    Format: Exactly pre-weighed tablets
    Composition:
    Phosphate Buffer
    300 mM Sodium Chloride  
    50 mM Sodium Phosphate
    Elution Buffer
    300 mM Sodium Chloride
    300 mM Imidazole
    50 mM Sodium Phosphate
    Volume:
    Phosphate Buffer: 400 ml
    Elution Buffer: 100 ml
    pH 8.0 ± 0.05 at 22°C
    Shelf life:
    Phosphate Buffer: 5 years after production date
    Elution Buffer: 3 years after production date
    Article No Product name Pack Size Solution Vol Price
    09-1010-10  IMAC phosphate buffer 10 tablets  400 ml/tablet  480
    09-1010-50  IMAC phosphate buffer 50 tablets  400 ml/tablet  975
    09-1012-10  IMAC elution buffer 10 tablets  100 ml/tablet  570
    09-1012-50  IMAC elution buffer 50 tablets  100 ml/tablet  1620