CATALOG #:
 
 
E3980  0.1 ml immunogen affinity purified immunoglobulin from
rabbit serum in 10mM PBS/1% BSA buffer pH 7.4 with
0.1% sodium azide.  
E3984  1 ml immunogen affinity purified immunoglobulin from
rabbit serum in 10mM PBS/1% BSA buffer pH 7.4 with
0.1% sodium azide.    
E3981  7 ml prediluted antibody in a 50mM Tris/1% BSA buffer
pH 7.6 with 0.1% sodium azide.  

INTENDED USE:  For Research Use Only.  Not for use in diagnostic procedures.  
CLONE:  N/A
IMMUNOGEN:  A synthetic peptide from the C-terminus of human Claudin-4.
IG ISOTYPE:  Rabbit IgG
EPITOPE:  C-terminus
MOLECULAR WEIGHT  22-24kDa
SPECIES REACTIVITY:  Human (tested).  Mouse (by sequence homology). Others not tested. 
DESCRIPTION:  Claudin 1-8 proteins are a family of transmembrane proteins associated with tight junctions. Tight
junctions are specialized regions of cell to cell contact; made up of a network of strands to act as a
molecular gasket for preventing the leakage of ions, water etc. between cells. They are abundant
in luminal epithelial sheets where they maintain epithelial cell polarity. Different tissues exhibit
different claudin composition.
APPLICATIONS:  Immunohistochemistry (IHC)
IHC PROCEDURE:  Specimen Preparation:  Formalin-fixed, paraffin-embedded tissues are suitable for use with this     
primary antibody.
Deparaffinization:  Deparaffinize slides using xylene or xylene alternative and graded alcohols.
Antibody Dilution:  If using the concentrate format of this product, dilute the antibody 1:200. The
dilutions are estimates; actual results may differ because of variability in methods and protocols.
Antigen Retrieval:  Boil tissue sections in 10mM Citrate buffer, pH 6.0 for 10 min followed by
cooling at RT for 20 min.   
Primary Antibody Incubation:  Incubate for 10 minutes at RT.
Slide Washing:  Slides must be washed in between steps.  Rinse slides with PBS/0.05% Tween.
Visualization:  Detect the antibody as instructed by the instructions provided with the visualization
system.    
POSITIVE CONTROL:  Tonsil and ovarian carcinoma
CELLULAR LOCALIZATION:  Cell membrane and cytoplasmic 
STORAGE & STABILITY  Store at 2-8°C. Do not freeze. The user must validate any other storage conditions. Every antibody is expiration dated. When properly stored, the reagent is stable to the date indicated on the label.
Do not use the reagent beyond the expiration date. 
There are no definitive signs to indicate instability of this product; therefore, positive and negative
controls should be tested simultaneously with unknown specimens. 
WARNINGS & PRECAUTIONS:  
1.  Avoid contact of reagents with eyes and mucous membranes.  If reagents come into
contact with sensitive areas, wash with copious amounts of water.
2.  This product is harmful if swallowed.  
3.  This product contains sodium azide, a chemical highly toxic in pure form.  At product
concentrations, though not classified as hazardous, sodium azide may react with lead and
copper plumbing.  Upon disposal, flush with large volumes of water to prevent metal azide
buildup in plumbing.  
4.  Consult local or state authorities with regard to recommended method of disposal.  In the
European Union, this material and its container must be disposed of as hazardous waste.
5.  Avoid microbial contamination of reagents.