• 产品编号:ANT-245
    • 产品名称:小鼠抗人波形蛋白抗体/Vimentin Antibody
    • 产品规格:500ug/1mg
    • 价  格:3500.00/5600.00/
    • 供 应 商:ProSpecbio
    • 库  存:
  • Catalogue Number ANT-245
    Type Mouse Anti Human Monoclonal Antibody.
    Introduction Vimentin expression in human malignant glioma cells depends on cellular density, algorithms of drug delivery and chemo/radio treatment. Vimentin and detyrosinated microtubules provide structural support for the extensive microtentacles observed in detached tumor cells and a mechanism to promote successful metastatic spread. Primary colorectal carcinomas display aberrant expression of vimentin, and have activated Notch and TGFbeta signaling pathways. Vimentin is a strong arterial substrate for transglutaminases. Transglutaminase-mediated vimentin dimerization results in a novel unifying pathway by which vasodilatory and remodeling responses may be regulated. Ablation of vimentin expression inhibits migration and invasion of colon and breast cancer cell lines.
    Clone hVimentin.
    Immunogen Purified Human Vimentin.
    Ig Subclass mouse IgG1.
    Purification Method Ion exchange column.
    Protein concentration 1mg/ml in PBS (after reconstitution).
    Shipping Conditions Antibody is shipped lyophilized at ambient temperature.
    Storage Procedures In lyophilized form, for long periods, store at 4°C in a dry environment. After reconstitution, if not intended for use within a month, aliquot and store at -20°C.
    Reconstitution Reconstitute with H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use. Stability / Shelf LifeTwo years lyophilized, one month in solution at 4°C.
    Titer For intra-cellular staining, use 5-10µl/106 cells. Wash twice with PBS/Azide before adding the appropriate amount of secondary fluorescent reagent.
    Applications Direct ELISA, Western Blot, Immuneprecipitation, immunohistochemistry.
    Cross Reactivity Vimentin antibody will recognize most mammalian Vimentin except mouse and rat.
    Other Available Forms This antibody is also available conjugated to biotin and FITC.
    Usage ProSpecs products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.